WPI PZMIV User Manual download pdf (Page 10)

229

MICROSCOPES, CAMERAS

Maintenance of the desired

temperature set-point can

now be achieved through the

use of a precision, shielded

temperature probe coupled to

a peripheral Air-Therm heating

unit.

Precision: Noise is a major

concern in any experimental

application. WPI's Air-Therm™

is electrically quiet as well

as acoustically quiet). Since

the Air-Therm can be placed

away from the incubator,

any residual electrical and

vibrational interference

from the heater is virtually

eliminated. Accuracy of ±0.1°C

can be routinely maintained at

the sample site (using a fully

programmable differential

algorithm to automatically

adjust the temperature on

the Air-Therm unit). Air-

Therm maintains a uniform

temperature across the

microscope stage (allowing for

uniform heating of multiwell

dishes). These tight tolerances

result in minimal focal drift

once sample/incubator equilibrium has been achieved.

Glass Bottom Dishes: For high numerical aperture (NA) live-cell

imaging, cells must be plated on glass (e.g., WPI’s FluoroDish Glass

Bottom Dishes, spotting 0.17 mm glass thickness) or standard cover slips.

Since cover slips inherit other problems associated with sterility and

accidental damage to expensive microscope condensers, WPI designed

new and improved glass bottom dishes (35 mm x 10 mm or 35 mm X

22 mm) that are not cytotoxic. A special adhesive in conjunction with an

advanced dish design makes the FluoroDish the only non-cytotoxic glass

dish in the world. (See page 210.) This means that cells can actually be

cultured for 4 to 5 days in the same dish and then carry the complete

cell culture with media from the incubator directly into the (Air-Therm

controlled) recording chamber. This is a comfort level that hitherto was

unattainable by scientists and clinical researchers since cells will have

to be transported from the culture dishes on to cover slips just prior to

imagery or attaching patch electrodes.

Chamber: The most problematic aspect of any live cell imaging

procedure or patch clamp approach is the thermal drift of high

magniﬁcation lenses caused by temperature ﬂuctuations in the

microscope's environment. It is necessary to constantly readjust the focus

during image acquisition due to this thermal-drift, introducing artifacts

during quantitative microscopy due to the photo-bleaching that occurs

during refocusing. The optimal solution to this problem is to use WPI’s Air-

Therm temperature controller connected to a Plexiglas or acrylic chamber

that encloses the entire imaging area (or at least the objectives and the

stage). Using such a chamber will result in highly stable temperature

control and minimal thermal drift.

Precision Heater Controller

Smart, electrically quiet air heater for live cell

imaging systems and custom incubators

AIR-THERM SPECIFICATIONS

AIR FLOW RATE 20 to 50 CFM (0.55 to 1.4 cubic meter/M)

CONTROL TEMP. RANGE Ambient to 60°C

TEMPERATURE RESOLUTION 0.1 °C

TEMPERATURE ACCURACY 0.2 °C

CONTROL MODES (a) Auto (PID set manually, or Self-Tune)

(b) Manual tune

ANALOG OUTPUT 0.5°C resolution

FOR CHART RECORDER 0-10V, 0 to 100 C (default, adjustable via

controller)

HEATING VOLUME More than 4 cu ft (85 liter), non-circulating

POWER (CE) 300 W, 95-135 V or 220-240 V, 50/60 Hz

DIMENSIONS 61⁄2 x 8 x 71⁄2 in. (15.5 x 21 x 19 cm)

SHIPPING WEIGHT 10 lb (4.5 kg)

Prices shown are in U.S. dollars. Actual charges will vary because of import duty, freight, and currency fluctuations. To obtain an exact quotation, contact your WPI office.

World Precision Instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com • Internet: www.wpiinc.com

AIRTHERMY-ATX Air-Therm Heater 120 V, U.S.

AIRTHERMZ-ATX Air-Therm Heater 240 V Specify line cord

2 pieces of 4.5 ft clear, coil-reinforced heater hose included with Air-Therm.

Specify Line (Mains) Voltage

OPTIONAL ACCESSORIES

15590 Clear hose, 2.5” diam., 4.5 ft

300276 Replacement platinum temperature probe

3491 5 ft (1.5m) probe extension cable

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WPI PZMIV User Manual Page 10

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