WPI PZMIII User Manual download pdf (Page 13)

MICROSCOPES, CAMERAS

172

FluoroDish

™

Cover-glass bottom for

observing and growing cells

in imaging related research

WPI’s FluoroDish™ tissue culture dishes

are now available in a larger range of sizes

and coatings. These polycarbonate dishes

provide exceptional imaging quality for many

applications requiring the use of inverted

microscopes such as high resolution image

analysis, microinjection and electrophysical

recording of fluorescent-tagged cells. Taking

advantage of WPI’s extensive experience with

low toxicity adhesives, FluoroDish uses a

specially formulated adhesive that is optically

clear, durable and with extremely low toxicity.

Tests by an independent laboratory have shown

that the 96-hour surviving rate of embryos is

100% when kept in FluoroDish, substantially

better than some other brands. The bottom

glass has superior UV transmission (30%

transmission at 300 nm, compared to less

than 7% for the most popular German glass).

Stringent quality control ensures that glass

thickness stays within the 0.17 ±0.01 mm

range.

Conventional plastic dishes and chambers

limit the utility of the inverted scope for many

applications because the thick plastic bottom

requires a long working distance objective

available only in lower magnifications. Each WPI

dish has a flat (0.17 mm thick) optical quality

glass bottom, allowing the use of a much

shorter objective working

distance, larger numerical

aperture (NA), and a

higher magnification

(up to 100x). The

larger NA and higher

magnification provide

superior quality imaging

for both classical

and fluorescence

microscopy. Higher

effective NA yields

brighter images for fluorescence and higher

resolution in Image Analysis. The glass bottom

permits the use of immersion objectives with

medium such as water, glycerin or oil for the

highest magnification possible. To optimize heat-

exchange, WPI’s glass-bottom dish is designed

to be flush (flat) with the microscope stage or

heating unit, therefore eliminating the air gap

that exists with modified plastic dishes in which

a glass cover slip has been inserted.

Three different sizes of FluoroDish are offered,

one type of 50 mm diameter dish and two

types of 35 mm diameter dishes. An inner well

is created within the dish by the glass bottom

and the tissue culture grade polystyrene which

forms the sides of the dish. All WPI dishes have

the advantages of low toxicity and good UV

transmission bottom glass. They are individually

packed and gamma sterilized.

The 35 mm dish has outside dimensions

similar to that of a Corning 35 mm dish

and has ø23.5 mm glass window (FD35)

or ø10 mm glass window (FD3510). Most

heaters and perfusion adapters designed

for the Corning 35 mm dish will also fit this

dish. The 23.5 mm glass window dish is

available uncoated, poly-D-lysine-coated, or

collagen-coated. Certain types of cell lines

(e.g., PC3 and HEK) adhere well to the uncoated

glass bottom dish. The poly-D-lysine coating

has been reported to improve the adhesion

of neuron cells, and type I rat tail collagen

has been reported to improve the adhesion of

muscle cells. The users can also apply to the

uncoated dish any special coating that is best

for their cell line.

The 10 mm glass window dish (FD3510) has

low sidewall for easy microelectrode access and

low solution volume. The low microelectrode

access angle is the lowest among all of 35 mm

dishes on the market (very close that of a 50

mm dish). The dish needs only 100 ~ 200 mL

to cover the bottom well, an important feature

when using expensive drugs and chemicals.

The 50 mm dish (FD50) has a large growth

area (35 mm well diameter), a low access angle

for microelectrodes, and grips for easy handling.

● Optical quality glass bottom

for better imaging quality

(RI=1.525)

● Low sample volume for

expensive chemicals

● Lowest access angle for

micropipette

● Low toxicity adhesive for

embryo research

● Choose from poly-D-lysine or collagen coated, or uncoated

Glass Ø

Access

Angle

Part Number ID (mm OD (mm) Glass Ø (mm) height (inside) Height (outside) Access Angle

FD35 33 35.5 23.5 7.8 9 29°

FD3510 10 35.5 10 1.5 4.65 17°

FD5040 47.5 49.82 35 7.25 7.4 17°

Prices shown are in U.S. dollars. Actual charges will vary because of import duty, freight, and currency fluctuations. To obtain an exact quotation, contact your WPI office.

UK: Tel: 01438-880025 • wpiuk@wpi-europe.com Germany: Tel: 030-6188845 • wpide@wpi-europe.com China: Tel: 21 68885517 • chinasales@china.wpiinc.com

FD35-100 FluoroDish Sterile Culture Dish, clear wall, 35 mm, 23 mm well, box of 100

FD35COL-100 FluoroDish Sterile Culture Dish, Collagen Coated, clear wall, 35 mm, 23 mm well, box of 100

FD35PDL-100 FluoroDish Sterile Culture Dish, Poly-D-Lysine Coated, clear wall, 35 mm, 23 mm well, box of 100

FD3510-100 FluoroDish Sterile Culture Dish, clear wall, 35 mm, 10 mm well, low sidewall, box of 100

FD5040-100 FluoroDish Sterile Culture Dish, clear wall, 50 mm, 35 mm well, box of 100

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WPI PZMIII User Manual Page 13

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